Vorum Silver Staining Protocol

• Fix gel 2 hrs (or ON) in
• 50 ml MeOH
• 12 ml HAc
• 1.35 ml 37% FA
• 37 ml H₂O

 
• Wash 3x in 35% EtOH (105 ml EtOH, 195 ml H₂O)

 
• Sensitize for 2 min in
• 100 ml H2O
• 200 ul 10% Na₂S₂O₃ (stored at -20C)

 
• wash 3 x 5 min in H₂O

 
• Stain 20 min in
• 0.2 g AgNO₃
• 200 ul 37% FA
• 100 ml H₂O

 
• Wash 2 x 1 min in H₂O

 
• Develop in
• 6 g Na2CO3
• 130 ul 37% FA
• 4 ul Na₂S₂O₃
• 100 ml H₂O

 
• Stop for 20 min in
• 50 ml MeOH
• 12 ml HAc
• 38 ml H₂O

 
• Store at 4 C in 1% HAc